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mouse mab against cgrp  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology mouse mab against cgrp
    Mouse Mab Against Cgrp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+mab+against+cgrp/CGRP+Antibody/pm38496903-81-58-64
    Average 94 stars, based on 166 article reviews
    mouse mab against cgrp - by Bioz Stars, 2026-10
    94/100 stars

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    Incubation:

    Article Title: Activation of the BMP2-SMAD1-CGRP pathway in dorsal root ganglia contributes to bone cancer pain in a rat model.
    Article Snippet: .. The samples were blocked for 1 h in PBS containing 0.3% Triton-X 100 and 5% normal donkey serum, then incubated overnight with rabbit polyclonal antibody (pAb) against BMP2 (1:500, ab14933; Abcam), mouse monoclonal antibody (mAb) against NeuN (1:1000, ab104224, Abcam), mouse mAb against GFAP (1:500, GB12096, Servicebio, Wuhan, China), rabbit pAb against NeuN (1:500, GB11138, Servicebio, Wuhan, China), mouse mAb against CGRP (1:500, sc-57053; Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit mAb against phospho-Smad1 (Ser463/465) (1:800, #13820; Cell Signaling Technology), or rabbit pAb against c-Fos (1:500, GB11069, Servicebio, Wuhan, China). .. The samples were washed in PBS the following day and treated for 1 h in PBS containing the relevant secondary antibody (1:500) from Cell Signaling Technology (catalog nos.

    Article Title: Activation of the BMP2-SMAD1-CGRP pathway in dorsal root ganglia contributes to bone cancer pain in a rat model
    Article Snippet: .. The samples were blocked for 1 h in PBS containing 0.3% Triton-X 100 and 5% normal donkey serum, then incubated overnight with rabbit polyclonal antibody (pAb) against BMP2 (1:500, ab14933; Abcam), mouse monoclonal antibody (mAb) against NeuN (1:1000, ab104224, Abcam), mouse mAb against GFAP (1:500, GB12096, Servicebio, Wuhan, China), rabbit pAb against NeuN (1:500, GB11138, Servicebio, Wuhan, China), mouse mAb against CGRP (1:500, sc-57053; Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit mAb against phospho-Smad1 (Ser463/465) (1:800, #13820; Cell Signaling Technology), or rabbit pAb against c-Fos (1:500, GB11069, Servicebio, Wuhan, China). .. The samples were washed in PBS the following day and treated for 1 h in PBS containing the relevant secondary antibody (1:500) from Cell Signaling Technology (catalog nos.



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    mouse mab against cgrp  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology mouse mab against cgrp
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    Fig. 2. Innervation of <t>CGRP</t> + nociceptive nerve fibers increased in the affected tibias during BCP progression. (A) Immunoflu- orescence analysis of CGRP + nociceptive nerve fibers (red) in the tibias on day 21 after Walker256 cell inoculation. Scale bars, 50 µm.
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    Real-time PCR analysis of <t>CGRP</t> <t>and</t> <t>COX-2</t> mRNA expression in the infrapatellar fat pad and synovial tissue of knee OA patients. a CGRP and b COX-2 mRNA expression in the infrapatellar fat pad and synovial tissue of knee OA patients. *Statistically significant difference between IPFP and SYN ( p < 0.001). All data are presented as the mean ± standard error ( n = 100)
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    Sequences of the primers used in this study
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    Fig. 2. Innervation of CGRP + nociceptive nerve fibers increased in the affected tibias during BCP progression. (A) Immunoflu- orescence analysis of CGRP + nociceptive nerve fibers (red) in the tibias on day 21 after Walker256 cell inoculation. Scale bars, 50 µm.

    Journal: Journal of integrative neuroscience

    Article Title: Netrin-1 Role in Nociceptive Neuron Sprouting through Activation of DCC Signaling in a Rat Model of Bone Cancer Pain.

    doi: 10.31083/j.jin2303047

    Figure Lengend Snippet: Fig. 2. Innervation of CGRP + nociceptive nerve fibers increased in the affected tibias during BCP progression. (A) Immunoflu- orescence analysis of CGRP + nociceptive nerve fibers (red) in the tibias on day 21 after Walker256 cell inoculation. Scale bars, 50 µm.

    Article Snippet: The samples were stained overnight at 4 °C with mouse monoclonal antibody against CGRP (1:100, sc-57053; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or rabbit polyclonal antibody against netrin-1 (1:100, PA5-95725, Invitrogen, Carlsbad, CA, USA).

    Techniques:

    Real-time PCR analysis of CGRP and COX-2 mRNA expression in the infrapatellar fat pad and synovial tissue of knee OA patients. a CGRP and b COX-2 mRNA expression in the infrapatellar fat pad and synovial tissue of knee OA patients. *Statistically significant difference between IPFP and SYN ( p < 0.001). All data are presented as the mean ± standard error ( n = 100)

    Journal: Journal of Orthopaedic Surgery and Research

    Article Title: Expression of calcitonin gene-related peptide in the infrapatellar fat pad in knee osteoarthritis patients

    doi: 10.1186/s13018-017-0568-1

    Figure Lengend Snippet: Real-time PCR analysis of CGRP and COX-2 mRNA expression in the infrapatellar fat pad and synovial tissue of knee OA patients. a CGRP and b COX-2 mRNA expression in the infrapatellar fat pad and synovial tissue of knee OA patients. *Statistically significant difference between IPFP and SYN ( p < 0.001). All data are presented as the mean ± standard error ( n = 100)

    Article Snippet: The sections were reacted with primary mouse monoclonal primary antibody against CGRP (Abcam) and rabbit polyclonal primary antibody against COX-2 (Abcam, Cambridge, MA) for 6 h at 4 °C.

    Techniques: Real-time Polymerase Chain Reaction, Expressing

    Correlation between CGRP and COX-2 mRNA expression levels in the infrapatellar fat pad and SYN tissues of knee OA patients. Correlation between CGRP and COX-2 mRNA expression levels in IPFP tissues harvested from 99 knees of OA patients. The values for one IPFP were removed as outliers

    Journal: Journal of Orthopaedic Surgery and Research

    Article Title: Expression of calcitonin gene-related peptide in the infrapatellar fat pad in knee osteoarthritis patients

    doi: 10.1186/s13018-017-0568-1

    Figure Lengend Snippet: Correlation between CGRP and COX-2 mRNA expression levels in the infrapatellar fat pad and SYN tissues of knee OA patients. Correlation between CGRP and COX-2 mRNA expression levels in IPFP tissues harvested from 99 knees of OA patients. The values for one IPFP were removed as outliers

    Article Snippet: The sections were reacted with primary mouse monoclonal primary antibody against CGRP (Abcam) and rabbit polyclonal primary antibody against COX-2 (Abcam, Cambridge, MA) for 6 h at 4 °C.

    Techniques: Expressing

    Relationship between CGRP and COX-2 mRNA expression level and K/L grade. a CGRP and b COX-2 mRNA expression in the infrapatellar fat pad of knee OA patients. All data are presented as the mean ± standard error

    Journal: Journal of Orthopaedic Surgery and Research

    Article Title: Expression of calcitonin gene-related peptide in the infrapatellar fat pad in knee osteoarthritis patients

    doi: 10.1186/s13018-017-0568-1

    Figure Lengend Snippet: Relationship between CGRP and COX-2 mRNA expression level and K/L grade. a CGRP and b COX-2 mRNA expression in the infrapatellar fat pad of knee OA patients. All data are presented as the mean ± standard error

    Article Snippet: The sections were reacted with primary mouse monoclonal primary antibody against CGRP (Abcam) and rabbit polyclonal primary antibody against COX-2 (Abcam, Cambridge, MA) for 6 h at 4 °C.

    Techniques: Expressing

    Sequences of the primers used in this study

    Journal: Journal of Pain Research

    Article Title: Increase and regulation of synovial calcitonin gene-related peptide expression in patients with painful knee osteoarthritis

    doi: 10.2147/JPR.S135939

    Figure Lengend Snippet: Sequences of the primers used in this study

    Article Snippet: The sections were then treated for 6 h at 4°C with rabbit polyclonal primary antibody against CD14 (Abcam, Cambridge, UK) and mouse monoclonal primary antibody against CGRP (Abcam).

    Techniques: Sequencing

    CGRP immunolocalization and mRNA expression in synovial tissue. Notes: Synovial tissue stained with ( A ) DAPI (nuclei), ( B ) CD14 or ( C ) CGRP. ( D ) Merged image. ( E ) High-magnification merged image of ( D ). Scale bars =100 μm. ( F ) Real-time PCR analysis of CGRP expression in non-cultured CD14− and CD14+ fractions. All data are presented as mean ± standard error (n=5). * p <0.05. Abbreviations: CGRP, calcitonin gene-related peptide; DAPI, 4′,6-diamidino-2-phenylindole; PCR, polymerase chain reaction.

    Journal: Journal of Pain Research

    Article Title: Increase and regulation of synovial calcitonin gene-related peptide expression in patients with painful knee osteoarthritis

    doi: 10.2147/JPR.S135939

    Figure Lengend Snippet: CGRP immunolocalization and mRNA expression in synovial tissue. Notes: Synovial tissue stained with ( A ) DAPI (nuclei), ( B ) CD14 or ( C ) CGRP. ( D ) Merged image. ( E ) High-magnification merged image of ( D ). Scale bars =100 μm. ( F ) Real-time PCR analysis of CGRP expression in non-cultured CD14− and CD14+ fractions. All data are presented as mean ± standard error (n=5). * p <0.05. Abbreviations: CGRP, calcitonin gene-related peptide; DAPI, 4′,6-diamidino-2-phenylindole; PCR, polymerase chain reaction.

    Article Snippet: The sections were then treated for 6 h at 4°C with rabbit polyclonal primary antibody against CD14 (Abcam, Cambridge, UK) and mouse monoclonal primary antibody against CGRP (Abcam).

    Techniques: Expressing, Staining, Real-time Polymerase Chain Reaction, Cell Culture, Polymerase Chain Reaction

    Effects of PGE2 on CGRP expression in cultured synovial fibroblasts and macrophages. Notes: Synovial fibroblasts and macrophages were stimulated in vitro with PGE2 (10 μM) or without PGE2 (vehicle) for 24 h prior to the extraction of total RNA for ( A ) CD14 and ( B ) CGRP expression analysis. All data are presented as mean ± standard error (n=5). * p <0.05. Abbreviations: PGE2, prostaglandin E2; CGRP, calcitonin gene-related peptide.

    Journal: Journal of Pain Research

    Article Title: Increase and regulation of synovial calcitonin gene-related peptide expression in patients with painful knee osteoarthritis

    doi: 10.2147/JPR.S135939

    Figure Lengend Snippet: Effects of PGE2 on CGRP expression in cultured synovial fibroblasts and macrophages. Notes: Synovial fibroblasts and macrophages were stimulated in vitro with PGE2 (10 μM) or without PGE2 (vehicle) for 24 h prior to the extraction of total RNA for ( A ) CD14 and ( B ) CGRP expression analysis. All data are presented as mean ± standard error (n=5). * p <0.05. Abbreviations: PGE2, prostaglandin E2; CGRP, calcitonin gene-related peptide.

    Article Snippet: The sections were then treated for 6 h at 4°C with rabbit polyclonal primary antibody against CD14 (Abcam, Cambridge, UK) and mouse monoclonal primary antibody against CGRP (Abcam).

    Techniques: Expressing, Cell Culture, In Vitro, Extraction

    CGRP expression in OA patients with strong/severe (VAS≥6) and mild/moderate pain (VAS<6). Notes: Real-time PCR analysis of CGRP expression in synovial tissue of OA patients. Box and Whisker plot for CGRP expression in synovial tissue of OA patients with strong/severe (VAS≥6; n=44) and mild/moderate pain (VAS<6; n=30). The plot shows median (bold horizontal line), IQR (box) and range, with outliers plotted beyond 1.5 times the IQR from the box. * p <0.05. Abbreviations: CGRP, calcitonin gene-related peptide; OA, osteoarthritis; VAS, visual analog scale; PCR, polymerase chain reaction; IQR, interquartile range.

    Journal: Journal of Pain Research

    Article Title: Increase and regulation of synovial calcitonin gene-related peptide expression in patients with painful knee osteoarthritis

    doi: 10.2147/JPR.S135939

    Figure Lengend Snippet: CGRP expression in OA patients with strong/severe (VAS≥6) and mild/moderate pain (VAS<6). Notes: Real-time PCR analysis of CGRP expression in synovial tissue of OA patients. Box and Whisker plot for CGRP expression in synovial tissue of OA patients with strong/severe (VAS≥6; n=44) and mild/moderate pain (VAS<6; n=30). The plot shows median (bold horizontal line), IQR (box) and range, with outliers plotted beyond 1.5 times the IQR from the box. * p <0.05. Abbreviations: CGRP, calcitonin gene-related peptide; OA, osteoarthritis; VAS, visual analog scale; PCR, polymerase chain reaction; IQR, interquartile range.

    Article Snippet: The sections were then treated for 6 h at 4°C with rabbit polyclonal primary antibody against CD14 (Abcam, Cambridge, UK) and mouse monoclonal primary antibody against CGRP (Abcam).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Whisker Assay, Polymerase Chain Reaction